Analysis of real time pcr amplification efficiencies from three genomic region of dengue virus. Report as inadecuate




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Silvana Vielma ; Daniel Atchley ; Guillermo Comach ; Alvaro Ramirez ; Saberio Pérez ; Luis Téllez ; Beatriz Quintero ; Erick Hernández ; Maritza Muñoz ; José Mendoza ;Investigación Clínica 2013, 54 1

Author: María Odreman-Macchioli

Source: http://www.redalyc.org/articulo.oa?id=372937691002


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Investigación Clínica ISSN: 0535-5133 riclinicas@gmail.com Universidad del Zulia Venezuela Odreman-Macchioli, María; Vielma, Silvana; Atchley, Daniel; Comach, Guillermo; Ramirez, Alvaro; Pérez, Saberio; Téllez, Luis; Quintero, Beatriz; Hernández, Erick; Muñoz, Maritza; Mendoza, José Analysis of real time PCR amplification efficiencies from three genomic region of dengue virus. Investigación Clínica, vol.
54, núm.
1, enero-marzo, 2013, pp.
5-19 Universidad del Zulia Maracaibo, Venezuela Available in: http:--www.redalyc.org-articulo.oa?id=372937691002 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Invest Clin 54(1): 5 - 19, 2013 Analysis of real time PCR amplification efficiencies from three genomic region of dengue virus. María Odreman-Macchioli1, Silvana Vielma1, Daniel Atchley2, Guillermo Comach3, Alvaro Ramirez4, Saberio Pérez1, Luis Téllez1, Beatriz Quintero1, Erick Hernández1, Maritza Muñoz1 y José Mendoza1. Laboratorio de Microbiología y Salud Pública del Estado Mérida, Universidad de Los Andes.
Mérida, Venezuela. 2College of Pharmacy Harding University, Searcy, Arkansas, USA. 3Laboratorio Regional de Diagnóstico e Investigación del Dengue y Otras Enfermedades Virales.
Maracay, Venezuela. 4Laboratorio Biología de Virus, Instituto Venezolano de Investigaciones Científicas (IVIC).
Altos de Pipe, Miranda, Venezuela. 1 Keywords: dengue virus, qPCR amplification, acute phase diagnosis, NS5 gene region. Abstract.
Early diagnosis of dengue virus (DENV) infection represents a key factor in preventing clinical complications attributed to the disease.
The aim of this study was to evaluate the amplification efficiencies of an in-house quantitative real time-PCR (qPCR) assay of DENV, using the non-structural...





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