Development of a new method for identification and quantification in cerebrospinal fluid of malignant cells from breast carcinoma leptomeningeal metastasisReport as inadecuate




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BMC Clinical Pathology

, 12:21

First Online: 12 November 2012Received: 09 July 2012Accepted: 02 November 2012

Abstract

BackgroundThe diagnosis of leptomeningeal metastasis LM in patients with solid tumors remains difficult. The usual diagnostic methods of cytomorphological assessment of cerebro-spinal fluid CSF and gadolinium enhanced MRI of the entire neuraxis lack both specificity and sensitivity. The Veridex CellSearch® technology has been designed for the detection of circulating tumor cells CTC in blood from cancer patients and validated for the follow-up and prognosis of breast, prostate, colorectal, and lung cancer. Our aim was to adapt this technology for the detection and the enumeration of tumor cells in the CSF of breast cancer patients presenting with LM.

MethodsOn the occasion of a randomized phase III study evaluating the role of the intrathecal treatment in LM from breast cancer DEPOSEIN, EudraCT N°: 2010-023134-23, the CellSearch® technology was adapted to direct enrichment, enumeration and visualization of tumor cells in 5 mL CSF samples, collected on CellSave® Preservative Tubes and analyzed within 3 days after CSF sampling.

ResultsSixteen CSF of 8 patients with primary breast cancer presenting with LM were studied. EpCAM+-cytokeratin + cells with typical morphology could be observed and enumerated sequentially with reproducible results in low or elevated numbers in 8 patients.

ConclusionThis methodology, established on a limited volume of sample and allowing delayed processing, could prove of great interest in the diagnosis and follow-up of cancer patients with LM, especially to appreciate the efficacy of chemotherapy.

KeywordsLeptomeningeal metastasis Neoplastic meningitis Meningeal carcinomatosis CellSearch® technology Immunomagnetic enrichment CSF cytology Electronic supplementary materialThe online version of this article doi:10.1186-1472-6890-12-21 contains supplementary material, which is available to authorized users.

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Author: Emilie Le Rhun - Frédéric Massin - Qian Tu - Jacques Bonneterre - Marcelo De Carvalho Bittencourt - Gilbert C Faure

Source: https://link.springer.com/







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