Screening of white-rot fungi manganese peroxidases: a comparison between the specific activities of the enzyme from different native producersReport as inadecuate




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AMB Express

, 2:62

First Online: 29 November 2012Received: 22 August 2012Accepted: 19 November 2012

Abstract

In this study manganese peroxidase MnP enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid media and a simplified process was established for the purification of extracellular enzymes.

Five lignin degrading organisms were selected Bjerkandera sp., Phanerochaete P. chrysosporium, Physisporinus P. rivulosus, Phlebia P. radiata and Phlebia sp. Nf b19 and studied for MnP production in small-scale. Extracellular MnP activity was followed and cultivations were harvested at proximity of the peak activity. The production of MnPs varied in different organisms but was clearly regulated by inducing liquid media components Mn2+, veratryl alcohol and malonate. In total 8 different MnP isoforms were purified.

Results of this study reinforce the conception that MnPs from distinct organisms differ substantially in their properties. Production of the extracellular enzyme in general did not reach a substantial level. This further suggests that these native producers are not suitable for industrial scale production of the enzyme. The highest specific activities were observed with MnPs from P. chrysosporium 200 U mg-1, Phlebia sp. Nf b19 55 U mg-1 and P. rivulosus 89 U mg-1 and these MnPs are considered as the most potential candidates for further studies. The molecular weight of the purified MnPs was estimated to be between 45–50 kDa.

KeywordsWhite-rot fungi Manganese peroxidase Lignin degradation Lignocellulose Enzyme purification Electronic supplementary materialThe online version of this article doi:10.1186-2191-0855-2-62 contains supplementary material, which is available to authorized users.

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Author: Juho Järvinen - Sanna Taskila - Ritva Isomäki - Heikki Ojamo

Source: https://link.springer.com/



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