On-line solid-phase enrichment coupled to packed reactor flow injection analysis in a green analytical procedure to determine low levels of folic acid using fluorescence detectionReport as inadecuate




On-line solid-phase enrichment coupled to packed reactor flow injection analysis in a green analytical procedure to determine low levels of folic acid using fluorescence detection - Download this document for free, or read online. Document in PDF available to download.

Chemistry Central Journal

, 6:155

Analytical Chemistry

Abstract

BackgroundAnalysis of folic acid FA is not an easy task because of its presence in lower concentrations, its lower stability under acidic conditions, and its sensitiveness against light and high temperature. The present study is concerned with the development and validation of an automated environmentally friendly pre-column derivatization combined by solid-phase enrichment SPEn to determine low levels of FA.

ResultsCerium IV trihydroxyhydroperoxide CTH as a packed oxidant reactor has been used for oxidative cleavage of FA into highly fluorescent product, 2-amino-4-hydroxypteridine-6-carboxylic acid. FA was injected into a carrier stream of 0.04 M phosphate buffer, pH 3.4 at a flow-rate of 0.25 mL-min. The sample zone containing the analyte was passed through the CTH reactor thermostated at 40°C, and the fluorescent product was trapped and enriched on a head of small ODS column 10 mm x 4.6 mm i.d., 5 μm particle size. The enriched product was then back-flush eluted by column-switching from the small ODS column to the detector with a greener mobile phase consisting of ethanol and phosphate buffer 0.04M, pH 3.4 in the ratio of 5:95 v-v. The eluent was monitored fluorimetrically at emission and excitation wavelengths of 463 and 367 nm, respectively. The calibration graph was linear over concentrations of FA in the range of 1.25-50 ng-mL, with a detection limit of 0.49 ng-mL.

ConclusionA new simple and sensitive green analytical procedure including on-line pre-column derivatization combined by SPEn has been developed for the routine quality control and dosage form assay of FA at very low concentration level. The method was a powerful analytical technique that had excellent sensitivity, sufficient accuracy and required relatively simple and inexpensive instrumentation.

KeywordsFolic acid On-line solid phase enrichment Flow injection analysis Cerium IV trihydroxyhydroperoxide Fluorescence detection Electronic supplementary materialThe online version of this article doi:10.1186-1752-153X-6-155 contains supplementary material, which is available to authorized users.

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Author: Samy Emara - Tsutomu Masujima - Walaa Zarad - Maha Kamal - Ramzia EL-Bagary

Source: https://link.springer.com/



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