Wounding, insect chewing and phloem sap feeding differentially alter the leaf proteome of potato, Solanum tuberosum L.Report as inadecuate




Wounding, insect chewing and phloem sap feeding differentially alter the leaf proteome of potato, Solanum tuberosum L. - Download this document for free, or read online. Document in PDF available to download.

Proteome Science

, 10:73

First Online: 26 December 2012Received: 07 August 2012Accepted: 22 December 2012

Abstract

BackgroundVarious factors shape the response of plants to herbivorous insects, including wounding patterns, specific chemical effectors and feeding habits of the attacking herbivore. Here we performed a comparative proteomic analysis of the plant-s response to wounding and herbivory, using as a model potato plants Solanum tuberosum L. subjected to mechanical wounding, defoliation by the Colorado potato beetle Leptinotarsa decemlineata Say, or phloem sap feeding by the potato aphid Macrosiphum euphorbiae Thomas.

ResultsOut of ~500 leaf proteins monitored by two-dimensional gel electrophoresis 2-DE, 31 were up- or downregulated by at least one stress treatment compared to healthy control plants. Of these proteins, 29 were regulated by beetle chewing, 8 by wounding and 8 by aphid feeding. Some proteins were up- or downregulated by two different treatments, while others showed diverging expression patterns in response to different treatments. A number of modulated proteins identified by mass spectrometry were typical defense proteins, including wound-inducible protease inhibitors and pathogenesis-related proteins. Proteins involved in photosynthesis were also modulated, notably by potato beetle feeding inducing a strong decrease of some photosystem I proteins. Quantitative RT PCR assays were performed with nucleotide primers for photosynthesis-related proteins to assess the impact of wounding and herbivory at the gene level. Whereas different, sometimes divergent, responses were observed at the proteome level in response to wounding and potato beetle feeding, downregulating effects were systematically observed for both treatments at the transcriptional level.

ConclusionsThese observations illustrate the differential impacts of wounding and insect herbivory on defense- and photosynthesis-related components of the potato leaf proteome, likely associated with the perception of distinct physical and chemical cues in planta.

KeywordsLeaf proteome Stress responses Insect herbivory Potato Solanum tuberosum L. Abbreviations2-DETwo-dimensional gel electrophoresis

IEFIsoelectic focusing

MALDI TOF MSMatrix-assisted laser desorption ionization time-of-flight mass spectrometry

Pin-IIPotato proteinase inhibitor II

PR proteinPathogenesis-related protein

RTReverse transcriptase

RubiscoRibulose-1,5-bis-phosphate carboxylase oxygenase

SDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresis.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5956-10-73 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Marc-Olivier Duceppe - Conrad Cloutier - Dominique Michaud

Source: https://link.springer.com/







Related documents