Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB-c miceReport as inadecuate




Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB-c mice - Download this document for free, or read online. Document in PDF available to download.

Parasites and Vectors

, 4:155

First Online: 05 August 2011Received: 17 June 2011Accepted: 05 August 2011

Abstract

BackgroundMore than 46 species of mammals can be naturally infected with Schistosoma japonicum in the mainland of China. Mice are permissive and may act as the definitive host of the life cycle. In contrast, rats are less susceptible to S. japonicum infection, and are considered to provide an unsuitable micro-environment for parasite growth and development. Since little is known of what effects this micro-environment has on the parasite itself, we have in the present study utilised a S. japonicum oligonucleotide microarray to compare the gene expression differences of 10-day-old schistosomula maintained in Wistar rats with those maintained in BALB-c mice.

ResultsIn total 3,468 schistosome genes were found to be differentially expressed, of which the majority 3,335 were down-regulated ≤ 2 fold and 133 were up-regulated ≥ 2 fold in schistosomula from Wistar rats compared with those from BALB-c mice. Gene ontology GO analysis revealed that of the differentially expressed genes with already established functions or close homology to well characterized genes in another organisms, many are related to important biological functions or molecular processes. Among the genes that were down-regulated in schistosomula from Wistar rats, some were associated with metabolism, signal transduction and development. Of these genes related to metabolic processes, areas including translation, protein and amino acid phosphorylation, proteolysis, oxidoreductase activities, catalytic activities and hydrolase activities, were represented. KEGG Kyoto Encyclopedia of Genes and Genomes pathway analysis of differential expressed genes indicated that of the 328 genes that had a specific KEGG pathway annotation, 324 were down-regulated and were mainly associated with metabolism, growth, redox pathway, oxidative phosphorylation, the cell cycle, ubiquitin-mediated proteolysis, protein export and the MAPK mitogen-activated protein kinases signaling pathway.

ConclusionsThis work presents the first large scale gene expression study identifying the differences between schistosomula maintained in mice and those maintained in rats, and specifically highlights differential expression that may impact on the survival and development of the parasite within the definitive host. The research presented here provides valuable information for the better understanding of schistosome development and host-parasite interactions.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-3305-4-155 contains supplementary material, which is available to authorized users.

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Author: Jinbiao Peng - Hongxiao Han - Geoffrey N Gobert - Yang Hong - Weibin Jiang - Xinzhi Wang - Zhiqiang Fu - Jinming Liu - Ya

Source: https://link.springer.com/



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