Three Phase Partitioning System, an Emerging Non-Chromatographic Tool for Proteolytic Enzymes Recovery and PurificationReport as inadecuate




Three Phase Partitioning System, an Emerging Non-Chromatographic Tool for Proteolytic Enzymes Recovery and Purification - Download this document for free, or read online. Document in PDF available to download.

* Corresponding author 1 INATAA, Bioqual Laboratory

Abstract : A rapid overview of the Three Phase Partitioning TPP system as an efficient non-chromatographic tool is given. This elegant non-chromatographic tool is able to purify numerous proteins and especially proteases in a one step protocol. TPP is able to do not only purify proteins, but also concentrate them into one of the phases and enhance their proteolytic activity. The application of TPP for the extraction and purification of plant milk-clotting enzymes and meat tenderizers agents are given. In addition, some proteases from other materials are summarized. This short communication stress showed that TPP is a simple, economical and quick method for proteases recovery from plant proteases. This elegant non-chromatographic tool may be performed in a purification process to be used successfully in food industries, namely to provide enzymes for cheese-making and meat tenderizing.

Keywords : Proteases Purification Three phase partitioning





Author: Mohammed Gagaoua - Kahina Hafid -

Source: https://hal.archives-ouvertes.fr/



DOWNLOAD PDF




Related documents