Cyanamide mode of action during inhibition of onion Allium cepa L. root growth involves disturbances in cell division and cytoskeleton formationReport as inadecuate




Cyanamide mode of action during inhibition of onion Allium cepa L. root growth involves disturbances in cell division and cytoskeleton formation - Download this document for free, or read online. Document in PDF available to download.

Planta

, Volume 234, Issue 3, pp 609–621

First Online: 15 May 2011Received: 02 March 2011Accepted: 28 April 2011

Abstract

Cyanamide is an allelochemical produced by hairy vetch Vicia villosa Roth

Its phyotoxic effect on plant growth was examined on roots of onion Allium cepa L. bulbs. Water solution of cyanamide 2–10 mM restricted growth of onion roots in a dose-dependent manner. Treatment of onion roots with cyanamide resulted in a decrease in root growth rate accompanied by a decrease in accumulation of fresh and dry weight. The inhibitory effect of cyanamide was reversed by its removal from the environment, but full recovery was observed only for tissue treated with this chemical at low concentration 2–6 mM. Cytological observations of root tip cells suggest that disturbances in cell division may explain the strong cyanamide allelopathic activity. Moreover, in cyanamide-treated onion the following changes were detected: reduction of mitotic cells, inhibition of proliferation of meristematic cells and cell cycle, and modifications of cytoskeleton arrangement.

KeywordsAllelopathy Cell division Cyanamide Microtubules Onion Root growth AbbreviationsAPXAscorbate peroxidase

BMMButyl-methyl methacrylate

BOA23H-benzoxazolinone

BSABovine serum albumin

CACyanamide

CahCyanamide hydratase

CATCatalase

DAPI2,4 Diamidino-2-phenylindole

DTTDithiothreitol

DWDry weight

FWFresh weight

GRGlutathione reductase

MSBMicrotubule stabilizing buffer

NBTNitroblue tetrazolium

PBSPhosphate buffered saline

ROSReactive oxygen species

RGRRoot growth rate

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Author: Dorota Soltys - Anna Rudzińska-Langwald - Wojciech Kurek - Agnieszka Gniazdowska - Elwira Sliwinska - Renata Bogatek

Source: https://link.springer.com/







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