Identification and characterization of six novel tetraspanins from Schistosoma japonicumReport as inadecuate




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Parasites and Vectors

, 4:190

First Online: 29 September 2011Received: 21 June 2011Accepted: 29 September 2011

Abstract

BackgroundTetraspanins TSPs, also known as members of the trans-membrane 4 super-family TM4SF, comprise an assemblage of surface antigens reported in eukaryotic organisms. In the work presented here, six novel TSP proteins from the human blood fluke Schistosoma japonicum S. japonicum were produced and analyzed through a combination of bioinformatics and experimental approaches.

ResultsSix novel TSP proteins of Schistosoma japonicum designated as Sj-TSP-#1~6 contained four trans-membrane regions and one large extracellular loop LEL with a conserved CCG motif. Size of the proteins varied from 227 to 291 amino acid residues. All the six proteins were produced in E.coli and immune sera to each protein were prepared. Analysis of transcription profiles of the proteins by RT-PCR showed that Sj-TSP-#4 was transcribed only in the egg stage while transcription of the Sj-TSP-#2 was detected in female worms but not in males. The similar results were obtained by Western blot. Immunolocalization of the TSP proteins by immunofluorescence assay showed that the Sj-TSP-#2, Sj-TSP-#5 and Sj-TSP-#6 were located in the tegument of worms.

ConclusionsThis study provided six novel TSP members of S. japonicum including their sequences and recombinant proteins. Availability of the novel proteins and information on their expression profile and location provided a basis for further investigation of the TSP proteins for their biological functions and as vaccine candidates.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-3305-4-190 contains supplementary material, which is available to authorized users.

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Author: Yanyan Jiang - Xindong Xu - Xiaoxing Qing - Weiqing Pan

Source: https://link.springer.com/







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