Phylogenetic groups and cephalosporin resistance genes of Escherichia coli from diseased food-producing animals in JapanReport as inadecuate




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Acta Veterinaria Scandinavica

, 53:52

First Online: 12 October 2011Received: 21 July 2011Accepted: 12 October 2011

Abstract

A total of 318 Escherichia coli isolates obtained from different food-producing animals affected with colibacillosis between 2001 and 2006 were subjected to phylogenetic analysis: 72 bovine isolates, 89 poultry isolates and 157 porcine isolates. Overall, the phylogenetic group A was predominant in isolates from cattle 36-72, 50% and pigs 101-157, 64.3% whereas groups A 44-89, 49.4% and D 40-89, 44.9% were predominant in isolates from poultry. In addition, group B2 was not found among diseased food-producing animals except for a poultry isolate. Thus, the phylogenetic group distribution of E. coli from diseased animals was different by animal species. Among the 318 isolates, cefazolin resistance minimum inhibitory concentrations: ≥32 μg-ml was found in six bovine isolates, 29 poultry isolates and three porcine isolates. Of them, 11 isolates nine from poultry and two from cattle produced extended spectrum β-lactamase ESBL. The two bovine isolates produced blaCTX-M-2, while the nine poultry isolates produced blaCTX-M-25 4, blaSHV-2 3, blaCTX-M-15 1 and blaCTX-M-2 1. Thus, our results showed that several types of ESBL were identified and three types of β-lactamase SHV-2, CTX-M-25 and CTX-M-15 were observed for the first time in E. coli from diseased animals in Japan.

KeywordsEscherichia coli food-producing animals cephalosporin resistance extended spectrum β-lactamase AmpC β-lactamase phylogenetic grouping AbbreviationsCEZcefazoline

CLSIClinical and Laboratory Standards Institute

ESBLextended spectrum β-lactamase

ESCextended-spectrum cephalosporinase

MICsMinimum inhibitory concentrations.

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Author: Tetsuo Asai - Kaori Masani - Chizuru Sato - Mototaka Hiki - Masaru Usui - Kotaro Baba - Manao Ozawa - Kazuki Harada - Hiro

Source: https://link.springer.com/







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