DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatmentReport as inadecuate




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Chromosome Research

, Volume 19, Issue 8, pp 955–967

First Online: 11 November 2011Received: 17 May 2011Revised: 14 September 2011Accepted: 15 September 2011

Abstract

Sequential immunolocalisation of 5-methylcytosine 5-MeC and fluorescence in situ hybridisation with chromosome-specific BAC clones were performed on Brachypodium distachyon mitotic metaphase chromosomes to determine specific DNA methylation patterns of each chromosome in the complement. In the majority of cells examined, chromosomes Bd4 and Bd5, which bear the loci of 5S and 35S ribosomal DNA, respectively, had characteristic 5-MeC patterns. In contrast, the distribution of 5-MeC along the metacentric chromosome pairs Bd1, Bd2 and Bd3 was more variable. There were numerous differences in distribution of methylated sites between homologous chromosomes as well as between chromosome arms. Some chromosome sites, such as pericentromeric regions, were highly methylated in all chromosomes. Additionally, the influence of a hypomethylating agent, 5-azacytidine, on B. distachyon chromosome methylation patterns was confirmed. It was found that some chromosome pairs underwent demethylation more easily than others, but there was no apparent regularity in demethylation of particular chromosome segments.

Keywords5-azacytidine Brachypodium distachyon Chromosome DNA methylation Epigenetics Abbreviations5-AzaC5-azacytidine

5-MeC5-methylcytosine

BACBacterial artificial chromosome

FISHFluorescence in situ hybridisation

NORNucleolus organiser region

LLong arm of the chromosome

rDNARibosomal DNA

SShort arm of the chromosome

Responsible Editor: Pat Heslop-Harrison.

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Author: Natalia Borowska - Dominika Idziak - Robert Hasterok

Source: https://link.springer.com/



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