Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp Cyprinus carpio L.Report as inadecuate




Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp Cyprinus carpio L. - Download this document for free, or read online. Document in PDF available to download.

AMB Express

, 1:41

First Online: 18 November 2011Received: 03 November 2011Accepted: 18 November 2011

Abstract

The microbes in the gastrointestinal GI tract are of high importance for the health of the host. In this study, Roche 454 pyrosequencing was applied to a pooled set of different 16S rRNA gene amplicons obtained from GI content of common carp Cyprinus carpio to make an inventory of the diversity of the microbiota in the GI tract. Compared to other studies, our culture-independent investigation reveals an impressive diversity of the microbial flora of the carp GI tract. The major group of obtained sequences belonged to the phylum Fusobacteria. Bacteroidetes, Planctomycetes and Gammaproteobacteria were other well represented groups of micro-organisms. Verrucomicrobiae, Clostridia and Bacilli the latter two belonging to the phylum Firmicutes had fewer representatives among the analyzed sequences. Many of these bacteria might be of high physiological relevance for carp as these groups have been implicated in vitamin production, nitrogen cycling and cellulose fermentation.

Keywordsintestinal tract biodiversity carp aquaculture pyrosequencing 16S rRNA Electronic supplementary materialThe online version of this article doi:10.1186-2191-0855-1-41 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Maartje AHJ van Kessel - Bas E Dutilh - Kornelia Neveling - Michael P Kwint - Joris A Veltman - Gert Flik - Mike SM Je

Source: https://link.springer.com/







Related documents