Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patternsReport as inadecuate




Serological testing of cattle experimentally infected with Mycoplasmamycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns - Download this document for free, or read online. Document in PDF available to download.

BMC Veterinary Research

, 7:72

First Online: 18 November 2011Received: 30 May 2011Accepted: 18 November 2011

Abstract

BackgroundTo study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony MmmSC, the agent of Contagious Bovine Pleuropneumonia CBPP, we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test CFT, a commercially available CFT, a competitive antibody ELISA cELISA and the immunoblotting test IBT. In addition, lung tissue samples were examined by culture.

ResultsA total of 89% 51-59 of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT 6 to 9 days post infection dpi, followed by IBT 9 to 13 dpi and cELISA 13 to 16 dpi. Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i early high responders, ii late high responders, and iii low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals.

ConclusionComparison of the assays- performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level.

AbbreviationsBCIP-NTB5-bromo-4-chloro-3-indolyl phosphate combined with nitrotetrazolium blue chloride

CFTcomplement fixation test

cELISAcompetitive enzyme linked immunosorbent assay, CBPP: Contagious Bovine Pleuropneumonia

dpidays post infection

IBTimmunoblotting test

kDakilodalton

MmmSCMycoplasma mycoides subsp. mycoides Small Colony

ODoptical densities

PCRpolymerase chain reaction

INH%percentage inhibition value

p.i.post infection

OIEOffice International des Epizooties World Organization for Animal Health

Electronic supplementary materialThe online version of this article doi:10.1186-1746-6148-7-72 contains supplementary material, which is available to authorized users.

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Author: Evelyn Schubert - Konrad Sachse - Jörg Jores - Martin Heller

Source: https://link.springer.com/



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