Binding of more than one Tva800 molecule is required for ASLV-A entryReport as inadecuate




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Retrovirology

, 8:96

First Online: 18 November 2011Received: 18 August 2011Accepted: 18 November 2011

Abstract

BackgroundUnderstanding the mechanism by which viruses enter their target cell is an essential part of understanding their infectious cycle. Previous studies have focussed on the multiplicity of viral envelope proteins that need to bind to their cognate receptor to initiate entry. Avian sarcoma and leukosis virus Envelope protein ASLV Env mediates entry via a receptor, Tva, which can be attached to the cell surface either by a phospholipid anchor Tva800 or a transmembrane domain Tva950. In these studies, we have now investigated the number of target receptors necessary for entry of ASLV Env-pseudotyped virions.

ResultsUsing titration and modelling experiments we provide evidence that binding of more than one receptor, probably two, is needed for entry of virions via Tva800. However, binding of just one Tva950 receptor is sufficient for successful entry.

ConclusionsThe different modes of attachment of Tva800 and Tva950 to the cell membrane have important implications for the utilisation of these proteins as receptors for viral binding and-or uptake.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-4690-8-96 contains supplementary material, which is available to authorized users.

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Author: Eleanor R Gray - Christopher JR Illingworth - John M Coffin - Jonathan P Stoye

Source: https://link.springer.com/







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