DNA-nanostructure-assembly by sequential spottingReport as inadecuate




DNA-nanostructure-assembly by sequential spotting - Download this document for free, or read online. Document in PDF available to download.

Journal of Nanobiotechnology

, 9:54

First Online: 18 November 2011Received: 15 September 2011Accepted: 18 November 2011

Abstract

BackgroundThe ability to create nanostructures with biomolecules is one of the key elements in nanobiotechnology. One of the problems is the expensive and mostly custom made equipment which is needed for their development. We intended to reduce material costs and aimed at miniaturization of the necessary tools that are essential for nanofabrication. Thus we combined the capabilities of molecular ink lithography with DNA-self-assembling capabilities to arrange DNA in an independent array which allows addressing molecules in nanoscale dimensions.

ResultsFor the construction of DNA based nanostructures a method is presented that allows an arrangement of DNA strands in such a way that they can form a grid that only depends on the spotted pattern of the anchor molecules. An atomic force microscope AFM has been used for molecular ink lithography to generate small spots. The sequential spotting process allows the immobilization of several different functional biomolecules with a single AFM-tip. This grid which delivers specific addresses for the prepared DNA-strand serves as a two-dimensional anchor to arrange the sequence according to the pattern. Once the DNA-nanoarray has been formed, it can be functionalized by PNA peptide nucleic acid to incorporate advanced structures.

ConclusionsThe production of DNA-nanoarrays is a promising task for nanobiotechnology. The described method allows convenient and low cost preparation of nanoarrays. PNA can be used for complex functionalization purposes as well as a structural element.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-3155-9-54 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Michael Breitenstein - Peter E Nielsen - Ralph Hölzel - Frank F Bier

Source: https://link.springer.com/







Related documents