Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgaeReport as inadecuate




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Biotechnology for Biofuels

, 4:61

First Online: 22 December 2011Received: 05 August 2011Accepted: 22 December 2011

Abstract

We describe a new selection method based on BODIPY 4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene staining, fluorescence activated cell sorting FACS and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.

Keywordsbiofuels BODIPY FACS lipid-rich strains microalgae isolation Electronic supplementary materialThe online version of this article doi:10.1186-1754-6834-4-61 contains supplementary material, which is available to authorized users.

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Author: Hugo Pereira - Luísa Barreira - André Mozes - Cláudia Florindo - Cristina Polo - Catarina V Duarte - Luísa Custódio -

Source: https://link.springer.com/







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