Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAsReport as inadecuate




Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs - Download this document for free, or read online. Document in PDF available to download.

Virology Journal

, 8:556

First Online: 22 December 2011Received: 08 June 2011Accepted: 22 December 2011

Abstract

BackgroundAvian leukosis virus ALV is a major infectious disease that impacts the poultry industry worldwide. Despite intensive efforts, no effective vaccine has been developed against ALV because of mutations that lead to resistant forms. Therefore, there is a dire need to develop antiviral agents for the treatment of ALV infections and RNA interference RNAi is considered an effective antiviral strategy.

ResultsIn this study, the avian leukosis virus subgroup J ALV-J proviral genome, including the gag genes, were treated as targets for RNAi. Four pairs of miRNA sequences were designed and synthesized that targeted different regions of the gag gene. The screened target i.e., the gag genes was shown to effectively suppress the replication of ALV-J by 19.0-77.3%. To avoid the generation of escape variants during virus infection, expression vectors of multi-target miRNAs were constructed using the multi-target serial strategy against different regions of the gag, pol, and env genes. Multi-target miRNAs were shown to play a synergistic role in the inhibition of ALV-J replication, with an inhibition efficiency of viral replication ranging from 85.0-91.2%.

ConclusionThe strategy of multi-target miRNAs might be an effective method for inhibiting ALV replication and the acquisition of resistant mutations.

KeywordsALV miRNA Inhibition Gag Multi-target series Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-8-556 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Qing-Wen Meng - Zai-Ping Zhang - Wei Wang - Jin Tian - Zhi-Guang Xiao

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents