Electrospun polyd-l-lactide-co-l-lactide hybrid matrix: a novel scaffold material for soft tissue engineeringReport as inadecuate




Electrospun polyd-l-lactide-co-l-lactide hybrid matrix: a novel scaffold material for soft tissue engineering - Download this document for free, or read online. Document in PDF available to download.

Journal of Materials Science: Materials in Medicine

, Volume 21, Issue 9, pp 2665–2671

First Online: 17 July 2010Received: 02 June 2010Accepted: 02 July 2010

Abstract

Electrospinning is a long-known polymer processing technique that has received more interest and attention in recent years due to its versatility and potential use in the field of biomedical research. The fabrication of three-dimensional 3D electrospun matrices for drug delivery and tissue engineering is of particular interest. In the present study, we identified optimal conditions to generate novel electrospun polymeric scaffolds composed of poly-d-l-lactide and poly-l-lactide in the ratio 50:50. Scanning electron microscopic analyses revealed that the generated polyd-l-lactide-co-l-lactide electrospun hybrid microfibers possessed a unique porous high surface area mimicking native extracellular matrix ECM. To assess cytocompatibility, we isolated dermal fibroblasts from human skin biopsies. After 5 days of in vitro culture, the fibroblasts adhered, migrated and proliferated on the newly created 3D scaffolds. Our data demonstrate the applicability of electrospun polyd-l-lactide-co-l-lactide scaffolds to serve as substrates for regenerative medicine applications with special focus on skin tissue engineering.

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Author: Petra J. Kluger - Ralf Wyrwa - Jürgen Weisser - Julia Maierle - Miriam Votteler - Claudia Rode - Matthias Schnabelrauch -

Source: https://link.springer.com/







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